Effect of Adenine Sulphates and Sucrose in Rooting of Vegetative Buds of Date Palm Phoenix dactylifera L. CV. Barhi Produced In vitro

Abstract

The current study was conducted at the Date Palm Research Center – University of Basrah from 25/1/2018 to 3/2/2019. It aims at examining the effect of Adenine Sulphates and Sucrose on the rooting of the vegetative buds of date palm cv. Barhi that are Produced in vitro. Four concentrations of Adenine Sulphates were used 30, 60, 90, 120 mg l-1 and two concentrations of Sucrose (30,45) g l-1 with the existence of 0.5 mg l-1 IBA. The nutrient medium of MS salts was used together with activated charcoal 250 mg l-1. The results showed a significant increase of the concentration 120 mg l-1 of Adenine Sulphates in the studied characters (the percentage of rooting buds and the numbers, lengths, and diameters of roots) which were scored as 87.5%, 2.5, 6.65 cm and 0.25 mm respectively. The concentrations 45 g l-1 showed a significant increase than the concentrations 30 in the percentage of the rooting buds as well as the number and length of the roots whose values were 83.6%, 2.25 and 5.32 cm respectively. The concentrations 30 g l-1 showed a significant increase than the concentrations 40 in the diameter of roots whose average was 0.20 mm. The results of the interaction showed a significant in cease in the interaction of (120 mg l-1 of Adenine Sulphates with 40 g l-1 of Sucrose) in the percentage of rooted vegetative buds, the number, and lengths of the other interactions whose values were 88.5%, 3 roots, and 6.8 cm respectively. In addition, the interaction of the same concentrations of Adenine Sulphates with 30 g l-1 sucrose showed a significant increase compared with the other interactions in the diameter of roots which was 0.25 mm.