Screening and DNA Methylation of Growth Hormone in Thyroidectomies Rats

Abstract

The goal of this research concerns with the methylation role for DNA of thyroidectomized lab rat. Thyroidectomy operation was applies on 40 lab rats. After 60 days of operation, these rats were injected with single dose/day of both GHL-T (2.5 mg/100 g Sc) and monkey anti-rGH serum (0.3 ml,ip). Deoxyribonucleic acid methylation of hypothalamic growth hormone gene expression was applied for lab animals subsequently by using northern blot analysis technique. The results of this experiment revealed significant correlation between thyroidectomy and decline in both thyroxine and thyroidiodotherionine values (p< 0.05) that’s affect GH value. Conclusion of this study proves that deoxyribonucleic acid methylation suppressing the feedback of growth hormone releasing hormone GRH to prevent physiological stress in thyroidectomized rats.