Bioavailability of Ellagic Acid in Plasma of 20 Healthy Volunteers Using HPLC Technique

Abstract

Ellagic acid (EA) and hydrolysable ellagitannins (ETs) were extracted from white flesh pomegranate; the ellagic acid was implicated with potent antioxidant, anticancer and antiatherosclerotic biological properties. ellagic acid act as excellent scavenger for chemical causing cancer , It form a layer on DNA to prevent free radicals damaging The concentration –time curve was constructed after administration of 80 mg ellagic acid to 20 healthy volunteers (14 male and 6 females) . The calibration curve for quantification of EA was linear (r2 = 0.9998) over concentration range from 400 to 6.25 ng/ml. the maximum concentration of ellagic acid (C max) was achieved in plasma of volunteers after 1 h (T max). the mean serum elimination half life was about 6.21 ± 1.35 h . The recovery of ellagic acid in plasma at different concentration from 50-400 ng/ml were between 101-117 % .