Determination and Validation of Tetracycline Residues in Poultry using High Performance Liquid Chromatography - Diode Array Detector Technique

Abstract

The aim of this work was to separate and determine the trace tetracycline residues in poultry chest, thigh and liver using High Performance Liquid Chromatography - Diode Array Detector (HPLC-DAD), with a mobile phase mixture consisting of acetonitrile: methanol: oxalic acid (0.01M) (25:15:60) and chromatographic column C8. The study was included 32 live poultries, which were received a chest injection of 1m of tetracycline standard solutions. Over four successive days, poultries were slaughter for analysis. The injection with 10×103 ppb of tetracycline showed that the traces of tetracycline residues exceeded the maximum residue limit (MRL = 200 ppb) in the thigh and chest meat in the 1st day and over first and second day of slay respectively. The traces of tetracycline have exceeded the value of (MRL = 600 ppb) in the liver over third and fourth day of slay. Limit of detection was LOD = 0.451 ppb, limit of quantification LOQ was 1.502 ppb, and recovery% of tetracycline at a concentration of 200.0 ppb was (88.966 - 91.055%) for Poultry chest, (84.623 - 87.667%) for thigh and of around (82.198 - 83.688%) for liver with a percentage relative standard deviations (RSD%) of < 1 %.