Association of MTHFR C677T Gene Polymorphism with Ischemia in Iraqi Population Undergoing Coronary Angiography

Abstract

Background: According to the last WHO report in 2014, Iraq is ranked as 22 out of 172 country in coronary artery disease (CAD) as the most leading cause of death. Iraq has an age standardized death rate of 187.65 due to CAD i.e., more than 187 per 100000 Iraqi people die due to CAD, which represent the first cause of death. To our knowledge, there is no Iraqi study concerning the association of MTHFR C677T gene polymorphism with sever stenosis.Objective: To study the link between C677T MTHFR gene polymorphism and coronary artery disease in individuals who had undergone coronary angiography in Iraqi people after we classify them into those with and without ischemia.Materials and Methods: population of the study comprised 150 patients (aged 50.4 ± 6.4 year) and 150 control subjects (aged 49.2 ± 4.6 year) that undergone to angiography for coronary vessels. We regarded angiography as positive when there is more than 70% reduction in the diameter of coronary vessels.Measurements of the participant's sugar and lipid profile were all carried out on fasting blood samples without anticoagulant by standard enzymatic assays. The MTHFR C677T polymorphism detection was carried out by PCR-RFLP method.Results: There is a significant statistical relationship between the MTHFR genotype and the presence of coronary artery disease (P< 0.001). In addition to statistically significant difference among the three genotypes CC, CT, TT regarding BMI, cholesterol level, triglycerides level, and VLDL (P < 0.05)Conclusion: MTHFR polymorphisms (CT, TT) genotype have been found to be risk factor for coronary artery disease in Iraqi population.