تأثير الوسط الغذائي والجزء النباتي والاوكسين في استحثاث الكالس من نبات الخشخاش Papaver somniferum خارج الجسم الحي

Abstract

A study was conducted in the tissue culture lab., College of Agriculture- University of Baghdad during 2006 and 2007. Supplemented with results indicated that no sign of contamination was recorded when seeds of Papaver somniferum were sterilized with 3% sodium hypochlorite (NaOCL) for 15 minuts. The results also showed that MS medium modified with 2 mg/L 2,4-D gave the highest values of fresh and dry weight callus (239.40, 22.50) mg respectively, compared with B5 medium supplemented with the same auxin concentration which gave (155.60, 14.80) mg for both fresh and dry weight callus respectively.MS medium supplemented with 3 mg/L 2,4-D was superior in both fresh and dry weight callus and gave (137.30, 13.10) mg respectively, compared with B5 medium at the same concentration of 2,4-D that gave (122.60, 11.80) mg for both fresh and dry weight callus respectively.The callus which was induced with 4 mg/L 2,4-D gave the highest values (39.60, 3.70) mg for both fresh and dry weight callus respectively, while MS medium supplemented with 4 mg/L of NAA gave (24.00, 2.10) mg for both fresh and dry weight callus respectively. Shoot tip was superior than cotyledons and hypocotyls in inducing callus initiation (90.3 8.5) mg and (69.1, 6.5) mg and (5.4, 0.5) mg for both fresh and dry weight callus inducted from shoot tip, cotyledons and hypocotyls respectively.