The Selective Pressure Effect of Antiseptics on the Patterns of Resistance in Staphylococcus aureus

Abstract

Isolates of Staphylococcus aureus had been collected from Education hospital of college of medicine /Babylon University by taking a swabs from hospital environment and patient ,then bacteriological and biochemical tests were performed to identify the bacterial isolates .All isolates of S. aureus were submitted to antibacterial susceptibility test using Ampicillin (AM), Methicillin (ME), Neomycin (N), Oxacillin (OX),and Vancomycin (VA). Mithacillin sensitive S.aureus) MSSA) were exposured to two types of disinfectant which used ordinarily in serial dilution from stock concentration of Iodine 2% and Pottasium permanganate 1g/10 ml .The results showed a wide spreading of S.aureus in the environment of Education hospital of college of medicine /Babylon University at rate reached to 38.88% and all isolates both Methicilline resistance S.aureus (MRSA) and Methicillin sensitive S.aureus (MSSA)were resist to Vancomycin at rate 42.85% , while the rate resistance of all isolates towards Ampicillin the was 100% , and the resistance to Oxacilline was disappear to all isolates at rate reached to 75%, these results were matched with resistance Neomycin.Additionally, The results in this study were revealed the iodine disinfectant at dilution 10-1 of (from the stock concentration 20%) was sufficient to kill the Methicillin sensitive S.aureus while, the same dilution of Potassium permanganate was insufficient to kill the bacteria by it's ability to grow in this dilution .