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Study of anti mutagenic and anti oxidant activity of clove plant extract in male white rats

دراسة النشاط المضاد للتطفير والأكسدة لمستخلص نبات القرنفل في ذكور الجرذان البيض*

ستار جاسم --- علي حمود --- رضية نجم عبد

journal of kerbala university مجلة جامعة كربلاء
ISSN: 18130410 Year: 2011 Volume: 9 Issue: 3 Pages: 345-358
Publisher: Kerbala University جامعة كربلاء

Abstract

This study was designed to test the antioxidant and antimutagenic activity of methanolic water extract of clove. The extract has been described by using thin layer chromatography TLC, the results which showed the presence of more than one component of this extract and when examining the activity of antioxidant by beta carotene spray process , It was observed appearance of the antioxidant activity in several bands . The antioxidant activity was determinated for these bands through the retention of the color yellow, which indicates the existence of effective antioxidant in some components of clove extract. When rats treated with 2 mg/kg of MMC lead to increased the level of DNA fragmentation and the interference treatment of extract at 500 mg/kg (with) and (after) the mutagen performed decrease the level of fragmentation,Thes results may indicate that the extract works as desmutagenic agent, while when given befor the MMC, the extract may works as antimutagenic agent. So the result of the oral gavage of mixing the extract with mutagen for five weeks reducing the level of the crash in the DNA compared with negative control. The results of MI of the interference treatment with extract (with) and (after) the mutagen performed a best result in the raising of median MI to 2.43 , 2.13 respectively compared with the positive control 1.16, while the extract before the treatment with mutagen did not posses any significant differences 1.68 compared with positive control .The result of the oral gavage of mixing the extract with mutagen for five weeks had been showed, in the first week led to raise the median MI. The result of chromosomal abnormality CA of mice stem cell possessed a high significant difference in the positive control 26.0 compared with the negative control 5.33, while in the interference between the extract and mutagen the result showed a decrease in the median of abnormality to both treatment (with) and (after), while the treatment ( before) did not posses any significant difference compared with positive control . Also the results of median chromosomal abnormality of the interference treatment (with) to five weeks showed a decrease in the median of CA. The results of this study refer to that the clove extract have antioxidant and antimutagenic activity In vivo and In vitro.

الخلاصةصممت الدراسة الحالية للكشف عن الفعالية المضادة للأكسدة والتطفير للمستخلص الميثانولي المائي لنبات القرنفل(clove) Syzygium aromaticum. فقد تم توصيف المستخلص النباتي باستخدام كروموتوغرافيا الطبقة الرقيقة TLC إذ بينت النتائج وجود أكثر من مكون للمستخلص وعند فحص الفعالية المضادة للأكسدة بطريقة الرش بالبيتاكاروتين لوحظ امتلاك المستخلص فعالية مضادة للأكسدة ظهرت في عدة حزم ، وقد حددت الفعالية المضادة للأكسدة لهذه الحزم من خلال احتفاظها باللون الأصفر. هذا وعند معاملة الجرذان بجرعة 2ملغم/كغم من عقار MMC لوحظ انه ادى الى زيادة مستوى تحلل اﻟـDNA ، كما ادى اعطاء مستخلص القرنفل بتركيز 500 ملغم/كغم مع وبعد المطفر الى خفض مستوى التحلل، مايدل على ان المستخلص يعمل كمضاد للطفرة خارج الخلية بالدرجة الاولى ومضاد للطفرة داخل الخلية بالدرجة الثانية عند إعطائه قبل المطفر. كما تم اختبار معاملة المستخلص مع المطفر لمدد زمنية مختلفة وهي أسبوع واحد وأسبوعان وثلاثة أسابيع وأربعة أسابيع وخمسة أسابيع وذلك لمعرفة التأثيرات التراكمية للمستخلص في التقليل من أو منع فعل العقار، اذ لوحظ انخفاض مستوى التحلل خلال الاسابيع المتتالية ليصل الى السيطرة السالبة في الاسبوع الخامس. كذلك لوحظ ان معاملة الجرذان بالعقار ادى الى خفض معامل الانقسام MI 1.16 مقارنة بالسيطرة السالبة 3.2 . في حين ان اعطاء المستخلص مع وبعد المطفر ادى الى رفع قيم MI الى 2.43 ، 2.13 على التوالي بينما لم تشكل المعاملة قبل1.68 فرقا معنويا مع السيطرة الموجبة، وعند استمرار التجريع لخمسة اسابيع بالمستخلص مع المطفر لوحظ ارتفاع قيم MI وصولا الى السيطرة السالبة في الاسبوع الخامس. كما اشارت الدراسة الكروموسومية لنقي العظم الاحمر الى ارتفاع معدل التشوهات عند المعاملة بالمطفر . ولكن اعطاء المستخلص مع وبعد المطفر ادى الى خفض معدل التشوهات ، بينما المعاملة بالمستخلص قبل المطفر لم تعط فرقا معنويا مع السيطرة الموجبة ، اما التجريع المستمر فقد ادى الى خفض التشوهات الى حد السيطرة السالبة .لقد دلت نتائج الدراسة الحالية ان لمستخلص القرنفل فعالية مضادة للاكسدة والتطفير داخل وخارج الجسم.Abstract This study was designed to test the antioxidant and antimutagenic activity of methanolic water extract of clove. The extract has been described by using thin layer chromatography TLC, the results which showed the presence of more than one component of this extract and when examining the activity of antioxidant by beta carotene spray process , It was observed appearance of the antioxidant activity in several bands . The antioxidant activity was determinated for these bands through the retention of the color yellow, which indicates the existence of effective antioxidant in some components of clove extract. When rats treated with 2 mg/kg of MMC lead to increased the level of DNA fragmentation and the interference treatment of extract at 500 mg/kg (with) and (after) the mutagen performed decrease the level of fragmentation,Thes results may indicate that the extract works as desmutagenic agent, while when given befor the MMC, the extract may works as antimutagenic agent. So the result of the oral gavage of mixing the extract with mutagen for five weeks reducing the level of the crash in the DNA compared with negative control. The results of MI of the interference treatment with extract (with) and (after) the mutagen performed a best result in the raising of median MI to 2.43 , 2.13 respectively compared with the positive control 1.16, while the extract before the treatment with mutagen did not posses any significant differences 1.68 compared with positive control .The result of the oral gavage of mixing the extract with mutagen for five weeks had been showed, in the first week led to raise the median MI. The result of chromosomal abnormality CA of mice stem cell possessed a high significant difference in the positive control 26.0 compared with the negative control 5.33, while in the interference between the extract and mutagen the result showed a decrease in the median of abnormality to both treatment (with) and (after), while the treatment ( before) did not posses any significant difference compared with positive control . Also the results of median chromosomal abnormality of the interference treatment (with) to five weeks showed a decrease in the median of CA. The results of this study refer to that the clove extract have antioxidant and antimutagenic activity In vivo and In vitro.