Study some properties of the enzyme glucose Oazomez which are produced from local isolation of the bacteria Streptomyces sp. HM5

Abstract

- The enzyme converts Alklkozaazomez Alklkosaly Alfrcktoswazailosaly Zailuluz. This enzyme is produced from many microorganisms, especially bacteria, Streptomyces,it has won numerous studies of this enzyme in terms of production, purification and characterization because of its importance in the areas of multiple use in the food and pharmaceutical industries. This research aims to study some properties of the enzyme glucose Aizumirizalmntg of local isolation of the bacterium Streptomyces sp. HM5 and partly purified. These include properties study of molecular weight, pH optimum of efficiency and stability, optimum temperature for the effectiveness and consistency, calculate the activation energy and the viability of the enzyme to retain its effectiveness after treatment with varying thermal different account of some constants kinetic enzyme such as Vmax and the Km and study the privatization enzyme towards our glucose and fructose and Zailoz as controlled. Enzyme was used Alklocuzaazmez product of local isolation of the bacterium Streptomyces sp.HM5 and purified molecule. Has been appointed the molecular weight using gel filtration technology and electrical migration. Has been appointed as effective enzyme in a manner LUNEL using the optical absorption and the wavelength of 560 Nanometeromenha was to know the pH optimum and temperature optimum of the effectiveness of the enzyme, stability, and affinity enzyme of the substances (Alklkoz, fructose, Zailoz) results showed that the molecular weight of the enzyme of 178,000, and that the pH optimum of 8 and that the effective degree Aharah optimal effectiveness was 60 m. As that of the high affinity towards our Landzim Zailoz compared Elokoz and fructose. As the value of Km / Vmax for Zaaloz 47.41x10-6, while the glucose and fructose was 10.75x10-6 and 5.42x10-6 Alyaltoala. This study showed that the enzyme consists of two identical and it is oneof the enzymes of higher molecular weight and high affinity for the enzyme towards our Zail