استخلاص وتنقية انزيم اليوريز من بكتريا العنقوديات الذهبيةStaphylococcus aureus المعزولة من مرضى خمج المجاري البولية

Abstract

Thirty isolates of Staphylococcus aureus bacteria which were isolated from urinary tract infections (Nephrolithiasis, Pyelonephrosis) were obtained .These isolates had been tested for their production of urease enzyme on urease agar slant. Isolate no 5 had been chosen for being the most productive isolate for this enzyme which is considered to be at the top of virulence factors which are produce by many bacterial genus which caused urinary tract infections like Klebsiella , Staphylococcus and Proteus . Staphylococcus aureus comes to be at the top of infectious agents for this enzyme which plays a vital role in (colonization) , (nephrolithiasis ) and also the development of Pyelonephrosis This enzyme hydrolyzes urea to ammonia and carbamide which separates spontaneously into ammonia and carbonic acid .The production of enzyme is either inducible or constitutive .The methods sulphate ammonium precipitation and ions exchange chromatography had been used in the purification of this enzyme. The results have shown an increase in protein and an increase in specific activity to be 93.13 unit / mg and 4.1 fold , yield 47.45 % .