Sensitivity of Coagulase negative Staphylococci Isolated from Different Diseases Cases to Antibiotics

Abstract

A number of 265 sample taken from patients in this hospital and other patients who consult the clinics in it had been gathered from TIKRIT General Educational Hospital . . The samples were taken for the patients whom doctors thought them infected with Urinary Tract Infections , Wound & Burn Infections and Mid- ear Infections for males and females in varied ages during the period from the beginning of December 2006 till the beginning ofMarch2007. Isolates diagnosed depending on of the morphological criteria cultural and morphological and biochemical test and API Staph Ident system test . The results revealed 60% Coagulase negative Staphylococci , which shows 27 isolates (45% ) regards S. epidermidis , 13 isolates (21.66%) S. saprophyticus , 10 isolates (16.67%) S. xylosus , 3 isolates (5%) S. lentus ,two isolates (3.33%) S. heamolyticus and one isolate (1.67%) S. simulans and other one isolate of S. hominis . The research has chosen 13 types of the antibiotics concerning sensitivity tests which show that most effective antibiotic against the isolates covered by this study is Nitrofurantoin (95.83%) , then Amikacin (93.33%) , Novobiocin 90.83%) , Chloramphenicol (88.33%) , Rifampicin (79.16%) , Vancomycin (75.83%) , Ciprofloxacin (74.16%) , Clindamycin (72.5%) , Gentamycin (61.66%) , Penicillin G (49.16%) , Trimethoprim (49.16%) , Tetracycline (40%) and Cefoxitin (17.5%) .The research made a test for producing β-lactamase enzymes , that show 85% of isolates (of this study) were positive to this test because β-lactamase enzyme is one of the significant enzyme which are produced by bacteria since it improves the resistance of bacteria for antibiotics