Isolation and Identification of Bacillus sp. and study of the optimat conditions for the production to extracellar polysaccharide on sugar Beet Molasses Medium.

Abstract

This study included the isolation and identification of Bacillus sp. from the soil, production of polysaccharide from sugar beet molasses as a nutrient medium and from a standard medium was compared. Production of polysaccharide from molasses medium exceeded that produced from the standard medium, since the highest production from molasses was 3.53 g/l after 7 days . The effect of different molasses concentration and nitrogen sources on the growth and the production of extra cellular polysacchaide the highest production was 3.80 and 4.10 g/l which achived in molasses concentration 12% and in ammonium sulphate as a carbon and nitrgen source, respectively.