The evaluation of activity and asymetrieal isolation of Adenosine Diaminase in the healthy patints with Anemia and Rheumatiod Arthritis serum and the both diseases

Abstract

Adenosine deaminase is one of the important enzymes that catalysts the salvage purin base. The effect of divalent metals ( Mn+2 ,Ca+2 ,Fe+2 ,Cu+2, Co+2 , and Cd+2 ) on activity concentration(O.O1M) was studied. The result showed the metals (Mn+2, Ca+2 and Fe+2) have activation effect to ADA while the (Cu+2, Co+2 and Cd+2) have an inhibition effect tothe enzyme in normal and abnormal seum in addition to ADA isoenzymes. The present study showed that (Cd+2) is the potent inhibitor to ADA activity. ADA isoenzymes in normal and abnormal serum are isolated by using three types column chromatography (Sephadex G1OO, G200, Cellulose- DEAE).The result, there are two isoenzymes for normal, anemic and patient with Rheumatoid Arthritis serum (ADA1,ADA2) while there are three isoenzymes in serum in both.