SPECTROPHOTOMETRIC DETERMINATION OF SACCHARIN BY COUPLING WITH DIAZOTISED 4-NITROANILINE

Abstract

A spectrophotometric method is described for the determination of saccharin. The Intended reacts with diazotised 4-nitroaniline in alkaline solution to form a stable brown coloured azo dye. The absorbance is measured at 427 nm.Beer’s law is obeyed over the concentration range of 10  200 g of saccharin in a final volume of 25 ml, i.e. , ( 0.4  8 ppm ) with a molar absorptivity of 2.75104 1. mol  1 , cm  1 and Sandell sensitivity index is 0.0088 g. cm2 , a relative error of  0.541 to + 1.851 % and a relative standard deviation of a  0.35 to  2.2 %, depending on the concentration level. Further, the method requires neither temperature control nor solvent extraction