Efficiency of Some Chalcone Derivatives in the Growth and Differentiation of Lettuce Callus: I–Initiation and Growth

Abstract

The effect of four chalcones derivatives prepared locally on callus initiation and growth of lettuce plant (Lactuca sativa L. cv. Longiflora) were carried out. These compounds were added to the basal medium at concentration of 10-4, 10-6, 10-8 and 10-10 M alone or in combination with the addition of NAA and BA at concentration of 3X10-6 M and 4X10-6 M respectively. The results indicated that the addition of chalcones derivatives alone to MS medium induced shoots, roots and callus from explant to certain extent. It was found that the addition of NAA along with the compounds used enhanced greatly callus, shoots and roots formation particularly at concentration of 10-6 and 10-8 M. Also, the addition of BA instead of NAA, stimulated greatly the callus formation similar to that grown on standard medium, during growth period (60 days). However, the addition of BA to all media containing chalcone compounds induced greatly shoot formation only as compared with NAA