تأثير الـ AgNO3 والـ BA على ظاهرة التزجج Vitrification الذي يصيب الاجنة الجسمية لنخيل التمر Phoenix dactylifera L. صنف (خصاب) خارج الجسم الحي

Abstract

The present study was under taken at tissue culture Laboratory-Date palm research center of Basrah University from September 2004 to Augest 2005 to determine the effect of AgNO3 and BA on the vitrified embryo in date palm (Phoenix dactylifera L.) CV. "Khasab" In vitro. seven concentration of AgNO3 used (0, 2, 4, 6, 8, 10, 12)mg/L and two concentration of BA (0, 0.1)mg/L. The results were found the AgNO3 addition at a concentration of 8 and 10 mg/L led to significant increased in fresh weight of embryogenic callus with other tested concentration, the somatic embryos were produced in higher numbers at concentration of 8 mg/L of AgNO3 compared with other concentration whereas, 6 mg/L of AgNO3 led to the best growth for somatic embryos, it decreased the percentage of vitrification and increased the percentage of normal somatic embryos compared with other concentration. The control treatment led to lower resulted. The addition of (BA) at concentration of (0.1 mg/L) led to significant increase in fresh weight of embryogenic callus, the number of cylindrical embryos and the percentage of normal embryos. Results also showed that interaction between of AgNO3 at (6 , 8) mg/L and BA at (0.1) mg/L led to significant increased in fresh weight of embryogenic callus compared with other interaction,AgNO3 at (12)mg/L and BA at (0.1)mg/L led to significant increased the number of somatic embryos, while AgNO3 at (6 mg/L) and BA at (0.1 mg/L) led to significant increased the percentage of normal embryos was (88) compared with other interaction the control interaction led to lower resulted.