MOLECULAR DETECTION OF ENTEROTOXIN ( CYT K ) GENE AND ANTIMICROBIAL SUSCEPTIBILITY OF BACILLUS CEREUS ISOLATES FROM MILK AND MILK PRODUCTS

Abstract

164MOLECULAR DETECTION OF ENTEROTOXIN ( CYT K ) GENE AND ANTIMICROBIAL SUSCEPTIBILITY OF BACILLUS CEREUS ISOLATES FROM MILK AND MILK PRODUCTSMohammed H. Khudor, Basil A. Abbas, Ban M. S. SaeedDepartment of Microbiology, College of Veterinary. Medicine, University of Basrah,basrah,Iraq(Received 19 June 2012,Accepted 28 June 2012)Keywords; milk, Mannitol egg-yolk agar, chloramphenicol.ABSTRACTTwo hundred twenty seven samples of milk, soft cheese, curls cheese and yogurt were taken to isolate B. cereus. The percentage of these bacteria was 32.7 %, 16.6 %, 18 % and 26% in milk, soft cheese, curls cheese and yogurt, respectively. Mannitol egg-yolk agar (MYP) supplemented with polymyxin B sulfate is used for isolation. Identification of the isolates was done by biochemical tests. Screening of isolates by polymerase chain reaction ( PCR ) revealed the presence of diarrheal toxin cyt K gene in 87.09 % of isolates. Susceptibility of isolates to 7 antimicrobial agents revealed neomycin, chloramphenicol and gentamycin 100% for each, streptomycin 96% and erythromycin 93.5 % to be the most effective antimicrobial, while the highest resistance was noted against penicillin 100 %.