Hassan J Hasony --- Saad Abdulbaqi --- Rafan Oday Fahad

Basrah Journal of Surgery مجلة البصرة الجراحية
ISSN: 16833589 Year: 2011 Volume: 17 Issue: 1 Pages: 14
Publisher: Basrah University جامعة البصرة


Original ArticlesRafan Oday Fahad*, Saad Abdulbaqi# & Hassan J Hasony@*MBChB, MSc Microbiology. #MBChB, CABP, Department of Pathology. @ MPhil, PhD, Department ofMicrobiology, College of Medicine, University of Basrah, Basrah, Iraq.AbstractCervical cytobrush and Pap smears were collected from 103 women attending the outpatientdepartment at Basrah Maternity and children hospital during the period from October 2009 tillthe end of January 2010. DNA was successfully extracted from 91 cytobrush samples, amplifiedfor the detection of human papilloma viruses (HPVs) using GP5+/GP6+ primers, in addition totyping using type-specific primers for HPV-16 and HPV-18 genotypes.The overall HPV prevalence was 20.8% with the dominance of genotype 16 (36.6%) over thegenotype 18 (10.5%) and the presence of non-16, non-18 genotype(s) in 42.1% of all HPVpositive cases. Younger women aged 25 years or less were more infected (26.3%) with thedominance of genotype-16 (21.1%) and among women aged 36-45 years, the non-16/non-18genotype(s) were the more frequently observed (13.3%). Infection rates were more frequentamong women married above 30 years (33.3%) and those with more than one lifetime husband(28.6%). Women's husband with polygamy practice significantly covariate with HPV infections(P< 0.05).Significant association (P<0.001) was obvious between infection with any HPV and abnormalcytology. The non-16/non-18 genotype(s) were more involved (28%) whereas genotype-16 wasdetected more frequent (20%) than genotype-18 (4%). In addition mixed infection of both highrisk types (16 and 18) was limited only to women with abnormal Pap smears.In archival blocks, the presence of HPV- DNA was constant in pre-invasive sequamous cervicallesions as all blocks revealed a positive HPV-16 either alone or in combination with genotype-18.