Effect of Pomegranate extract on P53 expression in Cancer Cell line (Hep-2)

Abstract

Background: Pomegranate (Punica granatum) fruits are widely consumed as juice. The potent antioxidant and antiatheroscleroticactivities of pomegranate are attributed to its polyphenols.Aim: Pomegranate extract were evaluated for in vitro cytotoxic and its effect on the level of p53 in Hep2 (Human larynxepidermal carcinoma).Methods: Aqueous extraction of pomegranate was prepared and in vitro cytotoxicity and amount of p53 was evaluatedon cancer cell line Hep2 (Human larynx epidermal carcinoma).Results: The study showed that the pomegranate extract has greatest antiproliferative activity by inhibition proliferationfrom 13% to 73.9%, at 10 μg/ml and 24 hr period of exposure. Also, the study showed significant decrease of p53 intreated group in comparison to control.Conclusions: According to the in vitro antiproliferative activity, inhibition of p53 expression pomegramate extractsmay be possess a potent anticancer activity.