إنتاج مبيد حيوي من لقاح الفطر Beauveria bassiana (Balsamo)Vuillemin لمكافحة حشرة من الخوخ الأخضرMyzus persicae (Sulzer)

Abstract

This study presented as attempted to produce a biological control agent Beauveria bassiana ( Balsoma ) Vuillemin fungus against insects pests such as the green peach aphid Myzus persicae ( Sulzer ) the basic elements necessary for production of the bio – cidal agent were determined according to the recominded methods. The results showed that the best fermentation medium for growth of fungus B. bassiana , was consisted of two – extracts of potato and rice seeds , the concentration of fungal spores in volume unit (ml ) was 27.0x108 spore / ml , while it was redused to 22.0 x 108 spore / ml ,and 21.8 x 10 7 spore /ml ,in both media , potato extract and rice extract respectively . The efficiency of some substances such as ( Kaoline , Rice seeds , Calcium carbonate and Talic ) were tested as carrier substances to fungi ,It was found that the Kaoline was appeared abetter effective than the rice seeds with regard to the fungal concentration in weight unit ( gm ) , reached to17.0 x 10 7spore / gm , while it was reduced to 10.0x106 spore / gm , in rice seeds substance , while two – substances Calcium carbonate and Talic were failed as substances carrying fungal inoculum. The percentage of ( 100 ) ml inoculum / ( 100 ) gm Kaoline was showed high significant results of the concentrations of fungal in weight unit ( gm ) , reached to 17.5x10 7 spore / gm. The results of the laboratory and field – work evaluation referred to efficiency of the bio – cidal as follow : - 1 – There is no change on the concentration of the fungal spores in weight unit( gm )of bio- cidal during the assigned storing period which lasted for six – months of storing reached to 17.0 x 10 7 spore / gm , in the first step of production and its continue to the end of storage period . 2 – Whole concentrations of bio – cidal was appeared high efficiency on the average of mortalities of green peach aphid , Myzus persicae . 3 – The bio – cidal was proved to have a high ability to use as a biological control against green peach aphid , in field experiment which was resulted in (80.31%) a mortality , when was compared with control treatment (0 %)