Laparoscopic assisted colonotomy suture using evertion and inversion techniques in goats

Abstract

The aim of the study was to investigate colonotomy via hand assisted laparoscopy device and to evaluate the closure by using Connell's and horizontal mattress suture technique. Twelve adult healthy local breed goats were used. They were divided randomly into two equal groups, Connell group and horizontal mattress group. The operations were done under the effect of general anesthesia using xylazine 2% (0.05 mg/kg B.W.) and ketamine 5% (3mg/ kg B.W.) injected intramuscularly together. The enterotomy incision was closed by Connell's pattern in Connell's group and by interrupted horizontal mattress technique in horizontal mattress group using polyglycolic acid suture material (No.0) after exteriorized of part of colon laparoscopically. Each group was divided into two equal subgroups depended on follow up of histopathological examination at a periods of 5 and 15 days post- surgery. The results of clinical examination revealed that the activity and appetite of animals were return early to the normal level, also the physical findings returned to the normal level value with a short time after operation in both groups. The histopathological findings of two groups appeared that there was on an inflammatory phase at a period of 5 days post operation, but the granulation tissue and collagen fiber formation was more prominent in horizontal group compared with Connell's group, while at the 15 days post operation in both groups revealed that a granulation tissue in the incision site which covered by epithelial layer with thick fibrous connective tissue and presence of myofibroblast at the incision site. This indicated that the wound was in a mature phase which was clear in horizontal group than in the Connell's group.