Isolation and screening of Indole - acetic acid producing Azotobacte

Abstract

Sixteen bacterial isolates belonged to the genus Azotobacter were obtained from different agricultural areas cultivated by alfalfa in sacred Karbala and Babylon governorates and each the isolates were identify by gram stain , Catalase test , Oxidase test , Indole test , Motility test, Sugar fermentation test and growth ability in 1% NaCl , glycerol and 0.1% phenol . All the isolates were subjected to a screening program to test their abilities for indole- acetic acid (IAA) production using different incubation conditions . Results showed that, Azotobacter sp.8a was the highest producer isolate and chosen to be used for further studies