In vivo study of the effect of collagen protein coated implant as compared with implants coated with a mixture of partially stabilized zirconia and collagen on osseointegration

Abstract

Background: Biocompatibility of orthopaedic surgical implants with bone tissue allows adequate osseointegrationbetween the bone and implant. To achieve this, implants are coated with biocompatible materials Commerciallypure titanium is widely used as dental implant material because of its suitable mechanical properties and excellentbiocompatibility,and to enhance osseointegration of the implant,organic and inorganic materials are used as abioinert coating material.Aim of the study: Comparison of the influence of the implant coated by biological material(collagen),and implantcoated by both bioinert ceramic(zirconia) and( collagen), on osseointegration by immunohistochemical , andradiographical studies with mechanical test.Materials and Methods: Commercially pure Titanium(cpTi) implants, coated with PSZ powder by electrophoreticdeposition (EPD) method, collagen protein, and mixture of collagen and partially stabilized zirconia(PSZ) wereplaced in the tibias of( 12 )New Zealand white rabbits, immunohistochemical tests for detection of expression ofosteocalcin and growth hormone receptor were performed on all the implants of both control and experimentalgroups for(3days,1, 2and6weeks) healing intervals.Mechanical test (torque removal test) was performed as anindicator for the presence of osseointegration and as a test for the mechanical property of bone-implant interface.Results: have illustrated that removal torque mean values, in all studied groups (uncoated and coated with both PSZand collagen at (2&6weeks) healing intervals were increasing with advancing time (higher at 6 than otherperiods),also results have shown that positive reaction for osteocalcin(OC)& growth hormone receptoe(GHR) wasexpressed by osteoblast cells(OB)at implants coated with collagen and implants coated with zirconia andcollagen,indicating that bone formation &maturation was accelerated by adding biological materials as amodification modality of implant surface..Conclusion: It is concluded that coating of implant with collagen and coating with both PSZand collagen showed anincrement in osseointegration in short interval period.