Abstract

Acne vulgaris is one of the most common skin disorders in youth especially during the puberty. This in vitro study performed to determine the antibiotic sensitivity and honey effect on staphylococci in acne vulgaris. Samples were collected from acne lesions of one hundred youngsters (24 girls, 76boys) among Sciences College/ Diyala University students in the age range of (18-24) years old. The specimens were cultured individually on blood agar. The cultures were then overnight incubated under aerobic conditions. Bacteria were identified and their sensitivity to common antibiotics were evaluated according to the standard procedures by using Muller-Hinton agar. In aerobic culture of Acne vulgaris samples, Staphylococci species was isolated from (64%) of studied sample, S. epidermidis was isolated from (44%) and S. aureus from (20%) of the total study samples. Staphylococci were more frequently isolated from males (65.8%) than females (58.3%). Doxicyclin and Gentamycin were equally effective against isolated bacteria (75% sensitivity), followed by (71.9%). Tetracyclin was associated with 50% sensitivity. Ampicillin and cloxacillin were least effective associated with a sensitivity rate of 21.9% and 3.1% respectively. In general there were a statistically significant difference in antibiotic sensitivity of the tested antibiotics The findings showed that no important or statistically significant difference in lowest concentration of honey associated with bacterial inhibition was observed between the 2 Staphylococci species. Results of this study showed that gentamycin and doxicycilin was the most sensitive antibiotics in vitro for staphylococcus isolated from acne vulgaris. To achieve a better treatment, a combination of honey with other antibiotics may be more efficient. We suggest in vivo studies for better evaluation and treatment of acne patients.

The article was added to IASJ on 2013-04-02

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