Isolation and identification of fungi associated with chronic respiratory infections in human and bovine

Abstract

In order to determine the fungi that association with respiratory infections in human and bovine, sixty human sputum samples were collected from Al-Yarmook educational hospital, and fifty samples were collected from bovine which showed clinical signs of chronic respiratory disease from alkarhk slaughterhouse in Baghdad city during a period from January to April 2012. The isolation results in human showed that 34 out of 60 (56.66%) sputum were positive for fungal isolation. The highest percentage of infection was seen in January (66.66%), While the lowest percentage seen in March (42.85%). The main fungal species that isolated included Candida albicans 12 out of 34 (35.29%), followed by C,tropicalis 5 out of 34 (14.7%), Aspergillus niger and A.fumigatus 3out of 34(8.82%) for each one, A. flavus, Rhizopus spp, Mucor spp, Penicillium spp and Saccharomyces cerevisiae 2 out of 34 (5.88%) for each one as well as the lower percentage of Alterneria alternate 1 out of 34 (2.94%). The results also revealed 26 out of 50 (52%) bovine lung samples were positive for fungal isolatation, February expressed high fungal isolation (58.82%), while April showed low percentage of fungal isolation (40%), the main fungal isolates from bovine lungs included Candida spp in high percentage (38.46%) especially C.albicans (23.07%) followed by C.tropicalis and C.stellatoidea (7.69%) for each one, Aspergillus fumigatus and Rhizopus spp 3 out 26 (11.53%) for each one, Mucor spp and Penicillium spp 2 out of 26 (7.69%) for each one, as well as the lower percentage of Aspergillus niger, Botrytis aclada, Cladosporium herbarum, Fusarium spp, Cryptococcus neoformans and Alterneria alternate 1 out of 26 (3.84%).