Preparation of Diagnostic Monovalent Antisera Against Staphylococcus aureus


Background: Monovalent antibodies have a great values in the diagnosis and monitoring of infectious diseases.Objective: the present study an effort to prepare diagnostic monovalent antibodies against Staph. aureus in order to reduce time and cost of diagnosis in bacteriological and biochemical tests.Methods: Diagnostic monovalent antibodies were prepared against Staphylococcus aureus. Hyper antibodies were obtained by rabbit immunization with whole antigen of Staphylococcus aureus. Anti-serum was checked for the presence of antibodies that detected by slide agglutination and ELISA tests. Sera were purified from cross-reacted antibodies by absorption with whole antigens of other bacterial isolates (Staph. epidermidis, Pseudomonas aeruginosa, Escherichia coli and Proteus) that cross-reacted with Staph. aureus antibodies. Antibodies were purified by repeating absorption process, then checked by ELISA test to diagnose presence of cross-reacted antibodies.Results: Specific monovalent antibodies against Staph. aureus were tested with different bacterial isolates, it was high sensitive and high specific diagnostic properties.Conclusions: Monovalent antibodies could be used for rapid diagnosis instead of classical way of culture and biochemical tests.