تحفيز إنتاج بعض مركبات الأيض الثانوي في كالس نبات الحلبةTrigonella foenum- graecum L. بإضافة تراكيز من حامض الساليسليك خارج الجسم الحي.

Abstract

The effect of adding different concentrations of salicylic acid (0, 50, 100, 150 or 200 mg/l) on the production of some secondary metabolites in callus of Trigonella foenum- graecum L. plant was studied. The quality and quantity of phytochemicals were investigated using methanol extracts of hypocotyls seedlings in vivo, in vitro. Calli were analyzed using high performance liquid chromatography (HPLC). Callus was initiated on dissected hypocotyls explants using Murashige and Skoog (1962) (MS) medium supplemented with Benzyl adenine (BA) at 1.5 mg/l with 0.5 2،4-Dichlorophenoxy acetic acid (2،4-D). The same combination was used for callus maintenance. Results also showed an increase in alkaloids and steroidal sapinogen glycosides in methanol extracts of callus culture. Results showed that above concentrations caused a reduction in callus fresh weight. Results also showed that a significant increase in the contents of alkaloids derived from callus. The best concentration of salicylic acid stimulated the production was 150 mg/l for alkaloids reached to 19.92, 66.50, 142.23, 84.73 and 99.01 mg/g for Scopaletine, Choline, Trigonelline, Carpaine and Gentianine respectively, and Yamogenin reached 14.32 mg/g at the concentration 50 mg/l of salicylic acid.