Study the best extraction conditions for some Hydrocolloids of vegetable origin and diagnosis of sugary components and thier functional groups

Abstract

This study about extraction of gums from the powder of the following plants ( Okra , Carob bean seeds , flax seeds ), The conditions of gums extraction from it raw materials had been varied , the best conditions to extract the gums from okra was at pH 7, Time 40 mints , temperature 50 Cº, The extraction conditions for carob bean gums was at pH 4.6, 60 mints, 100 Cº . while the conditions in flax seed was at pH 8, Time 60 , 100 Cº. Functional group had been diagnoses using IR spectroscopy Has appeared peaks indicate the presence of aggregates functional characteristics of each of gum gums . Noted that the absorption spectrum of the okra gum, carob bean has largely similarity , While disagreed infrared spectrum of flax gum them , Despite the similarity of the gums in the emergence of three peaks have values close in the absorption spectrum gums studied. Diagnosed components in studied gums by using HPLC spectroscopy and has found 9 types of sugars , fructose , glucose , gluconic acid , Mannose , galactronic acid , xylose , arabenose , galactose , rhammnose , Except that okra gum has been free of Rhammnose