تأثير المستخلص الكحولي الخام لأوراق نبات السذب (Ruta chalepensis ) على الإستجابةالمناعية في الفئران البيض

Abstract

This study was conducted to evaluate the activity of hot methanolic extract of Ruta chalpensis leaves on immune response in albino mice. The extract was prepared by hot methanolic extraction, then the percentage for dry weight in percent for plant powder weight. Chemical detection (of tannins, resins, comarins, flavonoids, alkaloids, glycosides and Essential oils)were carried out . LD50 Value was detected in order to select the optimal concentrations and according three percentages were chosen (3,5,10)% of LD50 value, and these percentages represented the three concentration (58,88,176)mg/kg which were used in this study to avoid the plant cytotoxicity , in addition to the cytotoxic test on SRBCs for these selected concentrations. The results showed that the extract have no toxicity on SRBCs in the mentioned concentrations,the non specific immune response was detected and reflected by increasing in percentage of activation and phagocytic index of peritoneal phagocytes ,moreover, evoke of humoral immune response that dependes on B Lymphocyte and this reflected by elevating values of both Arthus reaction and C.F.T., in addition to the immune response that dependent on T Lymphocyte which appear through elevated of DTH value by increasing the extract concentration . All results were compared with positive control (animals are injected with MMC) and negative control (animals are injected with PBS) The results revealed strong correlation between most of the immunological test by measuring the (r) value.