Abstract

Abstract:This study was done from 3/7/2011 to 1/1/2012 , different clinical samples were collectedfrom (1235) patients that coming to the Specialized center for chest and respiratory diseases /Baghdad to investigate Mycobacterium tuberculosis and study their resistant to first and secondline antimicrobial agents by using Bactec 960.The results obtained from direct smear examination with Ziehl – Neelsen stain in (294)patients that indicated these patients are Infected with tuberculosis .These samples has beencultured on solid medium Lowenstein Jensen media (L.J.) and liquid medium ( middle brook 7H8broth base) in the Mycobacteria Growth Indicator Tubes (MGIT) using a device (BACTECMGIT 960 System).Results of (275) samples that represented (22.27%) from total samples wereshowed a significant real growth that belong to bacteria Mycobacterium tuberculosis.From (275) isolates tested to 1st line anti TB (streptomycin (SM), isoniazid (INH),rifampin (RMP) and ethambutol (EMB)), (196) isolates were resistant to the first lineantimicrobial agents that used in the treatment of tuberculosis by using Bactec 960. (125) isolates(63.77%) showed resistance to multiple antimicrobial drugs (MDR-TB).Furthermore, the sensitivity test was done on thirty isolates that characterized by multipledrug resistance criteria (MDR-TB) on second line antimicrobial agents to investigate ExtensivelyDrug-Resistant Tuberculosis(XDR-TB) between these isolates. The results showed that the bestantibiotics in their impact on the isolates (MDR-TB) is (PAS) P-aminosalicylic acid as thepercentage of resistance was (3.3%).

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The article was added to IASJ on 2013-11-19

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