Identification And Diagnosis Of Cutaneous Leishmaninsis By Polymerase Chain Reaction Assay (Pcr) In Najaf Alashraf Province

Abstract

Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR)technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical samplecollected from lesion exudates patients.Methodology: The (115) sample were examined by smear slid preparation culture on RPMI 1640 and NNNthen DNA isolation. The DNA of the promastigote were amplified by PCR including primers selected onrepetitive KDNA for identification of leishmania species.The data was analyzed by using frequency andpercentage.Results: The PCR result showed that two species of leishmania (L. major with 620 bp and L. tropica with 800bp) exist.Conclusion: It is concluded from the present study that the PCR technique has high specificity and sensitivityduring the differentiation between Cutaneous Leishmanial species in comparing with conventional methods.Recommendation: The PCR technique can be use it to diagnosis and differentiation between different type ofCutaneous Leishmaniasis.