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Effect of Some Antibiotics on Uropathogenic Escherichia coli and Detection of Some Virulence Factors

Haythem Ali Al-Sayigh --- Hiyam Faez Ahmed Al-Hasson --- Jwan Ahmed Ali

Medical Journal of Babylon مجلة بابل الطبية
ISSN: 1812156X 23126760 Year: 2013 Volume: 10 Issue: 3 Pages: 669-676
Publisher: Babylon University جامعة بابل

Abstract

In this study, three hundred urine samples were obtained from patients from both sexes suffering from urinary tract infections (UTIs), who have attending to Hilla Teaching Hospital, one hundred thirty four (134) male (44.6%) and one hundred sixty six (166) female (55.3%) with no age range. Out of the) 300(urine samples, only (47) (15.7%) isolates were found to be related to Escherichia coli. Hemolysin production by E. coli was studied and it was found that (19) (40.4%) out of (47) isolate of E. coli were able to produce hemolysin on blood agar as zone of hemolysis surrounding bacterial colonies, and other isolates of E. coli (28) (59.6%) have able to produce siderophore on M9 media. Also, the antibiotic sensitivity test was studied on (47) isolates of E. coli and the results show that these isolates were resistance to Clindamycin (97.8%), (63.8%) for Cefotaxime and Cefaxime, Norfloxacine (59.5%), Naldixic acid (51.0%), Gentamycin (45.4%) and Amoxillin (44.6%, and all isolates were sensitive to Amikacin and Nitrofuranation (100%), (85%) for Chloramphenicol, (59.5%) for Streptomycin and Amoxillin (57.4%)

تم في هذه الدراسه عزل 47 عزله من بكتريا القولون من 300 عينه من مرضى من كلا الجنسين يعانون من التهاب المجاري البوليه. وجد ان من 47 عزله E. coli فقط (19) (%40.4) لها القابليه على انتاج الهيمولاسين على وسط اكار الدم اما باقي العزلات (28) (%59.6) هي تمتلك نظام السايدروفورات. ايضا درست فعاليه المضادات الحياتية على جميع عزلات E. coli فوجدت انها مقاومه للكلندماسين بنسبة (%97.8 )، سيفاتاكزيم والسيفاكزيم بنسبه (%63.8)، نورفلوكساسين بنسبه (%59.5), (%51) كانت مقاومه للنالدكسك اسد، جنتاماسين بنسبة (%45.4) والاموكسلين بنسبة (%44.6). كما وجد ان جميع العزلات كانت حساسة للاميكاسين والنايتروفيورانيشن بنسبة (%100) ، (%85) كلومفينيكول ، (%59.5) ستربتوماسين والاموكسلين كانت نسبة الحساسيه (%57.4).