The Relationship between Erythrocytes Membranes Variables, Conductivity of Erythrocytes Suspension and Activity of Purified Erythrocyte Glutathione-S-Transferase Enzyme GST in Diabetic Patients

Abstract

This study was included 69 persons (26 patients with insulin dependence diabetes mellitus IDDM with mean ages ( 49.57.66) years , and 43 patients with non- insulin dependence diabetes mellitus NIDDM with mean ages (46.167.22) years , for patients admitted to Medical city of Margan in Hilla, Babylon Governorate, Iraq. and 25 healthy person with mean ages (30.724.46) years, as control group. The blood glucose, sera malondialdehyde (MDA), sera glutathione (GSH), glycated hemoglobin (HbA1c) and conductivity for erythrocyte suspension are measured and compare with control group. The purified enzyme activity glutathione S-transferase (GST) from erythrocyte using anionic exchanger was measured at 340 nm with using 1-Chloro-2,4-Dinitrobenzene (CDNB) as a substrate at 37 °C . The specific activity of enzyme was calculated .The levels of variables are contacted together with these relationships to the erythrocyte cell wall by comparing with control group.