دراسة إمكانية توظيف بكتريا Bacillus spp. في السيطرة على إصابة بذور بعض المحاصيل ألاقتصاديه بالفطر Aspergillus spp. والتلوث بسمومها في المخزن

Abstract

These study in cluded the possibility of producing lotion vital vaccine bacteria Bacillus subtilis in order to use and trate it commercially economically by the fermentation, after selecting the middle Altkhmra media and the development of bacteria and the use of calcium carbonate as a holder for the manufacture of the product vital, and a number of seed extracts as used (wheat, barley, maize) Kausat Tkhmrah for the development of a vaccine bacteria B.subtilis maize extract showed high efficiency compared to the rest as extracts the number of bacterial cells 810x2.15 colony formation units / ml, and calcium carbonate were efficient as the bearer of the vaccine bacteria B.subtilis as the number of bacterial cells 810x70 unit configured colony / and gave 100 per ml center 50gm material bearer the highest number of bacterial cells, amounting to 810x88 unit configured colony / ml, and when testing the efficiency of isolation of bacteria Bacillus subtilis in the inhibition of growth radiography for fungal Aspergilus flavus and Aspergillus parasiticus found that isolation B.subtilis BM1 is the most efficint competent than the rest of the isolates in the inhibition of both fungi by inhibition of 100%Storage experiments showed that the vital extract in protection of weate at grams was efficient for A.flavus and A.parasiticus in addition to the prevention of the grams from contamination from B1 .where this treatment uduced the contamintation percent age for 800 mg/kg used the control treatment down to 190 and 310 mg/kg from Aspergillus flavus+BM1 and Aspergillus parasiticus+BM1 treatment respectively