SALMONELLA SEROTYPES ISOLATED AND IDENTIFIED FROM LOCALLY WHITE SOFT CHEESE

Abstract

Fourty locally white soft cheese random samples were collected from different markets of Baghdad Algadeda city in order to investigate the presence of Salmonellae Spp. in cheese which produced and consumed locally in Baghdad. The samples were collected during the period from December 2011 to March 2012.The samples were directly transferred to the laboratory and analyzed immediately without further storage.The isolation and identification methods include: (pre-enrichment) culture stage by peptone water then (Selective enrichment) culture stage by selenite broth after that culturing on sold (Selective media) which was Bismuth Sulphate agar. The biotyping by using API strip according to the API 20E miniaturized identification system for Salmonella SPP.. The isolated Salmonella strains were transferred on Triple Sugar Iron agar to undergone stereotyping at the Institute of Public Health,Baghdad,Iraq.Data revealed that 2 out of the total 40 (5%) of the cheese samples were contaminated with Salmonella spp. Salmonella typhimurium was the only serotype that have been found.