Utilization of Sesaban for Preparation of Substrate to Develop two Strains of Agaricus bisporus

Abstract

A study has been carried out to use the vegetative part of the sesaban by a certain rates in preparation the substrate of the mushroom Agaricus bisporus. Through substrate preparation stages, the results showed an increasing of substrate temperature through the early six days of the fermentation process. The higher temperature recorded along fermentation duration was after 5 days from fermentation beginning for the B medium. (40% wheat straw, 20% horse manure, 30% sesaban, 5% calcium sulfate, 5% rock phosphate) Ammonia smell disappeared after 35 days from the fermentation starting date for the media A(45% wheat straw, 45% horse manure, 5% calcium sulfate, 5% rock phosphate), B and C(30% wheat straw, 45% horse manure, 15% sesaban, 5% calcium sulfate, 5% rock phosphate), while the duration increased to 39 days for the E medium(40% wheat straw, 20% chicken manure, 30% sesaban, 5% calcium sulfate, 5% rock phosphate). The lower average of C:N ratio for the medium after the fermentation and pasteurization process were 12.6:1 for the E medium then 12.7:1 for the F medium(30% wheat straw, 45% chicken manure, 15% sesaban, 5% calcium sulfate, 5% rock phosphate) while the C:N ratios significantly increased for the media B, D(45% wheat straw, 45% chicken manure, 5% calcium sulfate, 5% rock phosphate), C and A with the averages reached 14.8:1, 15.9:1, 16.9:1 and 24.1:1 respectively. The medium B reached the highest yield with the average of 855.33 gm6 kg-1 growth medium after 21 days from picking while the lowest yield was with the average of 284.49 gm 6 kg-1 growth medium for the E medium.The strain F599 was exceed with average yield 668.88 gm6 kg-1 growth media as compared with the C9 strain which reached average yield 536.49 gm 6kg-1 growth media. The medium B reached the highest number of fruit bodies which was 30.33 fruit bodies box-1 while the lowest number of fruit bodies was 13.33 fruit bodies box-1 for the medium E, the strain C9 was exceed as comparing with the strain F955 with the average of 26.21 and 23.05 fruit bodies box-1 respectively.