GENETIC VARIATION IN SUMMER SQUASH UNDER ELECTRIC SHOCK AND USING SSR MARKERS

Abstract

Carried out this research in University laboratories of Wroclaw Environment and life sciences in Poland of March 10 to June 5, in 2010. To defined the genetic variations for seven genotypes of Cucurbita pepo L. to include 3 superiors hybrids refer to A,B and C and 4 parents refer to 1,2,3 and 4,when exposed to alternating electric shock a magnitude of 0, 1, 3, 5, 7 and 9 amp. SSR markers showed genetic variation among the plants after measurement the genetic percentage. characterized by the severity of shock 5A as being of genetic distance was high with the control treatment in most genotypes reaching 74.4% in inbred line 3 and 71.8% in inbred line 4 and 89.7% in the hybrid A (2 × 1) and 66.7% in the hybrid B (3 × 1). Indicating that the treatment of seeds with electric shocks, led to the creation of genetic variations and these variations can be considered as good sources for subsequent breeding programs. This confirms the tree diagram of the genetic relationship which shows the presence of a genetic divergence between certain genotypes and the presence of a genetic affinity between other genotypes.