The Antibacterial Effect of Phytosterols Isolated from Echinops heterophyllus in Comparison with MEBO® and Standard Antimicrobial Agents.

Abstract

Abstract:This study investigated the types of phytosterols that exists in the aerial and root parts ofEchinops heterophyllus and explored the antibacterial effect of these phytosterols against somepathogenic bacteria in comparison with Moist Exposed Wound Ointment (MEBO®) andgentamicin.Plant was extracted and phytosterols were isolated and purified from the crudeextract of Echinops heterophyllus by using chemical method. Thin Layer Chromatography(TLC) detected the presence of steroids in the aerial and roots part and gas chromatographymassspectrophotometer GC/MS analysis was used to identify some of these compounds andamong them were beta-sitosterol and stigmasterol. Standard dilutions of the phytosterolsisolated from Echinops heterophyllus were made from 10-100%; its antibacterial effect had beenexamined by seeded agar method against Staphylococcus aureus and Pseudomonas aeruginosa.The effect of these phytosetrols against Staphylococcus aureus was more potent than againstPseudomonas aeruginosa.The minimum inhibitory concentration (MIC) was seventy mg/ml and the minimumbactericidal concentration (MBC) was eighty five mg/ml. This study illustrates that sitosterolisolated from Echinops heterophyllus has a greater inhibitory effect on the test bacteria thangentamicine and closer results to MEBO®. It can be concluded in this study is a good step toshow the types of phytosterols isolated from Echinops heterophyllus and the antibacterial effectof these phytosterols in vitro against Staphylococcus aureus and Pseudomonas aeruginosa.