TEST SOME LOCAL ISOLATES OF THE BACTERIOM Bacillus thuringiensis INSECT FIG MOTH Ephestia cautela (Lepidoptera: Pyralidae) REARED ON ARTIFICIAL DIET IN THE LABROTARY .

Abstract

This research carried out in the laboratory of Technical Institute / Musayyibin 2011، has been tested six isolates local bacteria Bacillus thuringiensis on thefirst stage and the last of the larvae of a figs moth Ephestia cautela(Lepidoptera: Pyralidae) reared on the artificial diets ، while treated control withwater only. ، And took the percentage of mortality results after 2، 5، 8 dayssince results showed that the highest mortality rate of 96.6% on the eighth dayin each of the isolates DE 1-8 and NA 2-1 and DE 2-3 when Concentration 1.2× 106 spore /ml. While less kill ratio at treatment SL 4-1 in the concentration of1.2 × 104 spore /ml، amounting to 23.3%. isolation DE 2-3 achieved the highestrate at 72.2% at the concentration 1.2 × 106 spore /ml. within eight days، whilethe lowest rate was 23.3% when isolation SL 4 -1 Concentration 1.2 × 104spore /ml. The higher efficiency relative to Bacillus thuringiensis bacteria in themortality of the last instar larvae of the insect fig moth Ephestia cautella ratehas reached 34.7% in isolation DE 2-3 focus 1.2 × 106 after the fifth day، whileless relative efficiency rate was 6.8% when SL 4-1 in isolation Focus 1.2 × 104.The overlap between the concentration and the periods of isolation has achievedhigher mortality in DE 2-3 at 46.8% at the concentration of 1.2 × 106 spore /mlafter 5 days of treatment either at least it was 0.00% in isolation SL 4-1 at theconcentration of 1.2 × 104 spore /ml after 2 days of treatment. As for the larvaethat has not reached the stage of the pupae is observed that the lowest ratecould not be reached 52.6 when isolation DE 2-3 focus 1.2 × 106 spore /ml .Which differed significantly from the treatment comparison، while thepercentage larvae that could not is when isolation SL 4-1 in Focus 1.2 × 104 at94%، which did not differ significantly from the treatment comparison، eitheradult that emerged from these pupae There was no significant effect onemergence percentage compared to the treatment of comparison where thelowest percentage rise of 88.8% when isolation AN 8-3 consecration 1.2 × 106spore /ml while did not differ the rest of the other treatments for the of controlmortality. The mortality efficiency was shown in the last larval instarcompared to the first .