PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals

Abstract

Multi drug resistant (MDR) and Extended Spectrum Beta Lactamase (ESBLs) of Pseudomonas aeruginosa were detected. Pseudomonas aeruginosa is a bacterium responsible for severe infections in burn’s units, plasmid DNA analysis and encoded many types of genes responsible for beta-lactamases. To determine the type of genes responsible for beta-lactam broad spectrum in P. aeruginosa strains isolated from 100 swabs of burn’s units environment, using a molecular methods (PCR) by primers specific to ESBLs (bla ) genes oxacillin hydrolyzing capabilities OXA-10, OXA-4 and Vietnam Extended-Spectrum β-Lactame VEB-1. The results revealed that 15 strains were isolated from burn units environment. All of 15 (100%) were positive OXA-10 and only one (6.6%) for OXA-4 while the other gene VEB-1 was found in 6 (40%) isolates.