An in-vitro scan electron microscope comparative study of dentine-Biodentine interface

Abstract

Background: This research was an in-vitro SEM comparative study of Dentine – Biodentine TM interface.Materials and Methods: Sixty three freshly extracted human molars, Biodentine TM (Septodont, France), MTA (ProRoot, Tulsa, Brazil), GIC (MediFil, Promedica, Germany), light microscope, scaler and pumice, high speed hand piece, diamond bur, Scan Electron Microscope: VEGA\ Easy Probe. TESCAN – Germany. The study was performed first at the University of Mosul, College of Dentistry to dental models were brought the sixty-three of the specialty dental health center in Mosul. The teeth was prepared by cleaning, cutting, and removing all the caries and examined under light microscope and decayed teeth was excluded .Then the teeth was divided randomly into three main groups (A, B, C) and each major group was divided into three sub groups: (A1, A2, A3) was filled with (Biodentine TM), (B1, B2, B3) was filled with (MTA) and (C1, C2, C3) was filled with the (GIC). Each subset contains seven (7) samples. All groups were filled according to the manufacturer instructions, and then restored at 37°C and 100% humidity. After storage periods of (7, 14, 28) days, the teeth were sectioned mesio-distaly using a low speed diamond saw (Isomet, Buehler Ltd.), and examined under SEM at the University of Technology-Nano Research Center in Baghdad.Results: Under the condition of this in vitro study, examination with SEM showed that the marginal gaps between the experimental materials and the dentine is time dependant, with the best results was observed between Biodentine and dentine interface.Conclusion: The marginal gaps between the experimental materials and the dentine are time dependent