INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS

Abstract

Twenty samples of Laryngeal and Tracheal tissues from laying chickens of twoflocks with suspicious infectious laryngeotracheitis were tested for the detection of thecausative virus by using virus isolation in chicken embryo fibroblast (CEF)cell cultureand chicken embryos by chorioallantoic membrane inoculation and conventionalpolymerase chain reaction(PCR).The virus was isolated from three samples (larynx andtrachea) out of ten samples at Al-Sawara city on (CEF)cell culture and produce pocklesions on chorioallantoic membrane of infected chicken embryos in which the virus wasisolated.Positive PCR results were detected in the three clinical samples, isolated virus inCEF cell culture and isolated virus in chicken embryos (inoculated on chorioallantoicmembrane).