The effect of chronic exposure to X-ray on blood parameter , hormones and female reproductive system of albino rats

Abstract

The aim of this study was to investigate the effect of chronic exposure of X-Ray on reproductive system in albino female rats . Twenty four adult of female rats were divided randomly into three groups (8 animals / groups), the first group considered as control group (G1) , Second group (G2) was exposed to X-Ray (80kv), along one meter/ daily for whole one month as well as the third group (G3) that had the same productive of (G2) but for two months, blood samples ovary were collected from the three groups , after the determined period . Significant decrease ( P <0.05) in the number of (W.B.C , RBC, PTL) and in level and Hb concentration in the blood serum of exposed animals in G2 and G3 as compared with G1. and Significant decrease ( P <0.05) in the number of (W.B.C , RBC, PTL) and in Hb level in G3 compared with G2. Significant decrease (P <0.05) in the concentration of Estrogen hormone (E2), Progestrone hormone in the blood serum of exposed animals of G2 and G3 as compared with G1. and no significant effect of X-Ray in Follicle stimulating ( FSH ) and Luteinizing hormone ( LH) in the blood serum of exposed animals of G2 and G3 as compared with G1. The results of histological section of ovary exposed animals for one month G2 ,to appear as blood congestion and inflammatory cells , comparing with control group G1 . while there was clear histological changes in rats exposed for two month G3 include the occurrence of destruction in Overian follicles, that lost their , degeneration , fat density and inflammatory cells in addition late effect of radiation injury include nicrosis and fibrosis, comparing with control group G1. As conclusion from the study that the chronic exposure of X-Ray radiation causes harmful effect on some blood and hormones parameters and ovary , tissue in female white rats.