The Effect of crude Terpenoids extract of Chrozophora tinctoria L. on some biological aspects of house fly Musca domestica L (Diptera: Muscidae) and isolation and identification of active compounds by using high performance liquid chromatograph

Abstract

Series experiment conducted to evaluate the activity of crude extract of terpenoid compound of Chrozophora tinctoria on mortality of egg ,larval instars and pupa of house fly Musca domestica, as well as the cumulative mortality of immature stages ,and isolation , identification of active compounds by using H.P.L.C technique . Egg mortality was 90% at concentration 1mg/ml compared with 0.4 % in the control treatment .The mortality of 1st,2nd 3rd of larval instars were (57.64,54.67,48.80) % respectively at concentration 1mg/ml compared with (0.8,0.6,0.8)% respectively in the control group , pupal mortality increases from 0.4 % in the control group to 18.06% at concentration 1mg/ml .Cumulative mortality was 100% for all concentration compared with 15% at the control group .The study included isolation and identification of five compounds from crude extract of terpenoid ,it was found Teter methyl hydrazine compound was retention time 1.333 mintue and was the first compound isolation