Study on Prevalence of Bovine Mastitis and Its Major Causative Agents in Salahadin City, Iraq.

Abstract

The Study have been done to know the most common causative agents which cause bovine mastitis in Salah Eldin City during the period from 1/1/2013 to13/12/2013. Diagnosis of the cases have been done for 200Milk samples from Clinical and Sub Clinical Mastitis cattle .by using California mastitis test (CMT) ,enzyme–Linked Immune sorbent assay, culture media ,biochemical test and the antimicrobial susceptibility of isolated bacteria was done to determine its susceptibility. A total of 200 cows were included in this study and out of these, 80 (40%) were found to be reactive by California mastitis test (CMT). Bacteriological analysis of milk samples collected simultaneously yielded positive result in 70(35%) of the samples, virological analysis of milk samples yielded positive result in 45 (22.5%), Fungi analysis of milk samples yielded positive result in 6(4.3) and finally Parasite detected in 4(2.9) .Bacteriological analysis carried out at the quarter level showed that obtain to 250 pathogens agents were positive for various bacteria , Viruses ,fungi and parasit. The predominant bacteria isolated were Staphylococcus species (47.11%), Streptococcus species (31.40%) and coli forms (9.92%). Other bacterial, fungi and parasite species were isolated at lower rates. The predominant virus were Bovine herpesvirus1and Bovine herpesvirus 4 while Food and Mouth Disease virus was lower rate. Antimicrobial susceptibility test showed that most of the isolates in the study area were found to be highly sensitive to cloxacillin, gentamycin and amoxicillin, and moderately sensitive to oxytetracycline. Nevertheless, Streptococcus ,Staphylococcus and coli forms species isolated were resistant to penicillin.