Anti-Neutrophil Antibodies (ANCA) Level in Psoriatic Patients with Different Degree of Severity

Abstract

Psoriasis is a chronic, autoimmune skin disease with cutaneous manifestations;association of several factors including environmental, genetics and immunologicalabnormalities with psoriasis is documented.The acronym ANCA (Anti-Neutrophil Cytoplasmic Autoantibodies) is defined by anaccumulation of autoantibodies with specificity against different granulocytic, monocyte andprobably endothelial cytoplasmic antigens include (elastase, lactoferrin, lysozyme andCathpsin G ), are most commonly found in systemic vasculitis, necrotizing vasculitis and inactive generalized wegener's granulomatosis.The objective of this study is to assess the anti-neutrophil antibody level (ANCA) asinflammatory markers in patients with psoriasis with different degree of severity, correlate theresults with the degree of severity and compare the level of these antibodies with those ofcontrol group.This study was conducted from Feb. 2014 to Feb. 2015, blood samples were collectedfrom thirty two persons attending to Al-Karama Teaching Hospital, Baghdad TeachingHospital and private clinics, sixteen of them having psoriasislabelled as study groups and therest were free of the disease labelled as control group. The psoriatic patients were classifiedinto three groups according to the degree of severity of the disease as mild (four patients),moderate (four patients) and sever (eight patients), the Psoriasis Area Severity Index (PASI)were used to assess the severity of the disease. Thepatient’sgroup distributed as male (31.2%)and female (62.8%), the serum level of ANCA antibody was measured in thepatient’s serumof different degree of severity and in control group by using EnzymeLinked ImmunosorbentAssay technique (ELISA) in laboratory of Al-karama Teaching Hospital.The results proved that the serum levels of ANCA in psoriatic patients weresignificantly higher than that of control group in all degree of disease severity when ANOVAtest used to analyze the data, also the results reported that the level of anti- elastase and antlactoferrinwere significantly higherin sever degree group of psoriatic patients than mild andmoderate degree groups (p≤0.05)when tested by LSD test, no significant difference wasnoted between mild and moderate degree groups (p >0.05).From that we can conclude that autoantibodies against neutrophil antigens aregenerally associated with inflammatory psoriatic disorder; In addition, the autoantibodieslevels were related to the degree of severity of the disease especially antielastase andantilactoferrin