Study the efficiency of aqueous extracts of green tea and licorice on sperm parameters and reproductive hormone levels of male rats treated by Dimethoate

Abstract

The study aims to assessing the protective role of aqueous extract of green tea andaqueous extract of licorice in reducing toxicity of the pesticide Dimethoate on reproductiveefficiency of male rats and some fertility standards as a study of sperm standards andcalculate weights of the components of the reproductive system. In this experiment, (50)male from adult rats were randomly assigned to 5 equal groups, each group comprised (10)animals. Control group (C) was gavage with distilled water, The first group (T1) wasgavage with pesticide Dimethoate in concentration (12,5) mg /kg of body weight, Thesecond group (T2) was gavage with Dimethoate in concentration of (12.5) mg/kg andaqueous extract of green tea a concentration of 100 mg/kg of body weight, The third group(T3) was gavage with Dimethoate in concentration of (12.5) mg/kg and aqueous extract oflicorice concentration of 100 mg/kg of body weight, The fourth group (T4) was gavagewith Dimethoate in concentration of (12.5) mg /kg and aqueous extract of green tea aconcentration of 100 mg/kg and aqueous extract of licorice concentration (100) mg /kg ofbody weight. After 60 days the animals were weighed , At the expiration of the experiment,Which was lasted 60 days, Five animals were dissected after taking blood samples for thepurpose of obtaining serum for assess hormones levels Testosterone and stimulating follicle(FSH) and LH, Then animals were eradicated and testes and epididmis weighed, The tailof the epididymis was taken to study sperm parameters (concentration, movement, viable,normal), Five males have been isolated from each group randomly for the purpose ofconducting fertility test on them and to study the male's ability to fertilization and thecreation of pregnancy and to make sure the pregnancy and the number and weight ratiobirth. Statistical analysis showed a significant decrease (P <0.05) levels of hormones (T,FSH, LH) and a significant decrease (P <0.05) standards sperm studied for a pesticide (T1)compared with the control group and others groups whose results were mixed differencesnearby.According to this study, it can concluded that exposure to the pesticide Dimethoate causenegative changes in the concentrations of hormones and standards of sperm, and the studyproved that giving aqueous extracts of green tea and licorice together or each one has apositive role in reducing the toxic effects and physiological resulting from exposure toDimethoate.