PREVALENCE OF BRUCELLOSIS IN BUFFALOES OF BASRA GOVERNORATE, BASRA - IRAQ

Abstract

The present study was conducted to identify the prevalence of brucellosisamong buffaloes in Basra governorate, via examination of serum samples from 250she buffaloes reared in different Basra reigns. Sera were examined firstly by roseBengal test (RBT) followed by indirect enzyme linked immunsorbent assay (Elisa).The result of RBT indicated that from 250 buffaloes serum samples there were27(10.8%) positive against Brucella abortus antigen.Elisa test was performed on 88 sera samples that included a 27 RBT positivesera and other 61 negative sera, and the result revealed that 21( 23.8%) seropositivesera for Brucella abortus. According to the regions of Basra Governorate thepercentage rate of brucellosis were indicated in: Al Hartha 6(5,28%) then Al Qurna5(4,4%), Al Dear 4(3.5), Al Zubair 3(2.6%), Al Medaina 2(1.76%) and Al Tanooma1(0.88%). More over, infection in older animals found more significant ( P < 0.05)than in youngness, beside that infection rate were high in pregnant buffaloes incompared with non pregnant animals.Conclusion: the brucellosis of buffaloes in Basra governorate were caused by B.abortus and were more prominent in pregnant animals, therefore animals screeningof suspected animals was advised,