The Antimicrobial Activity of Peel and Seeds Extracts of Red Grapes

Abstract

Phenolic compounds in the grapes has multi-functional activities including anti-microbial activities. The present study assessed the antibacterial and anti-fungal activity of the locally available red grape which collected from local markets in Erbil city. The phenolic compounds were extracts from Seeds and Peels. Agar well diffusion method was utilized for the antimicrobial activity assessment. The results showed that the highest inhibition zone was observed by peel and Seed extracts against Bacillus cereus which were 13mm and 16mm respectively. In contrast the lowest inhibition zone was obtained from seed and peel extracts against Klebsiella Pneumoniae which were 3mm and 7mm respectively. Also the inhibition zone of peel and Seed for S. typhi were 7mm and 9 respectively. However, the grape extracts showed no inhibitory activity against Candida albicans and Sacchromyces cervisiae. Also the data showed that the highest minimum inhibitory concentration of the peel and seed extracts were against Escherichia coli which were 460 and 225 (mg/ml) respectively. Whereas the lowest minimum inhibitory concentration of the peel and peed extracts were against B. cereus which were 20 and 30 (mg/ml). In conclusion B. cereus seemed to be more sensitive for Peel and Peel extracts. Whereas, P. aeroginose and K. Pneumoniae showed the lowest sensitivity against seed and peel extracts. Furthermore, regarding the minimum inhibitory activity, the data showed that the gram positive bacteria were more sensitive to the seed and peel extracts. But, the fungal species seemed to be resisting the phenolic compounds in peel and seed extracts.