Effect of Olea Europea ( Olive oil ) on gentamicin induced hepatorenal toxicity in male rats

Abstract

Gentamicin is aminoglycoside antibiotic commonly used for the treatment ofGram-negative bacterial infection. In many cases, it has been the only effectivetherapeutic drug against bacterial strains resistant to other antibiotics, but nephrotoxicityand hepatotoxicity side effects limit its use. The aim of this study is to investigate theprotective effect of olive oil against gentamicin-induced hepatorenal toxicity in malealbino rats. In this study we used 24 wistar-albino rats and divided them into 4 equalgroups. Each experimental group consisted of six animals. group1,control they weregiven normal saline only ,group2,gentamicin 100 mg/kg/day intraperitoneal(IP),group3,olive oil 5 ml/kg/day (oral administation) ,group 4,gentamicin 100 mg/kg/dayintraperitoneal (IP) and olive oil 5 ml/kg/day (oral administration).Treatments wereadministered once daily for 21 days. After 21 days, biochemical and histopathologicalanalysis were conducted to evaluate hepatoranal toxicity. Serum levels of urea,creatinine, cholesterol,triglyceride,and activity of AST and ALT were measured.Animalstreated with gentamicin alone showed a significant increase in serum levels of thesemarkers.Treatment of rats with olive oil showed significant improvement in renal andhepatic function , presumably as a result of decreased boichemical parameters associatedwith gentamicin-induced hepatorenal toxicity.Histopathological examination of the ratskidneys and liver confirmed these observations. Therefore olive oil may protect againstgentamicin-induced hepatorenal toxicity